Development of drug candidates based on genomics and chemoinformatics against myocardial infarction: inhibitors of matrix metalloproteinases



Project Summary

Matrix metalloproteinases (MMPs) play an important role in tissue remodeling associated with various physiological and pathological processes such as morphogenesis, angiogenesis , tissue repair, arthritis, chronic heart failure, chronic obstructive pulmonary disease, chronic inflammation, and cancer metastasis. As a result, MMPs are considered as viable drug targets in the therapy of the above diseases. In spite of the high therapeutic potential of MMP inhibitors, all clinical trials except for doxycycline for periodontal diseases have failed so far. This can be attributed to 1. poor selectivity of the MMP inhibitors, 2. poor target validation for the targeted therapy, as well as 3. poorly defined predictive preclinical animal models for safety and efficacy. Lessons from previous failures and recent discoveries of oxidative/nitrosative activation and phosphorylation of MMPs, non-matrix related intra- and extracellular targets of MMP give new hope for MMP inhibitor development for both chronic and acute diseases.

Activation of MMP-2 plays an important role in cardiac ischemic injury and in the mechanism of cardiac stress adaptation. We have previously shown that in hyperlipidemic animals, which represent a high-risk group for ischemic heart disease, the adaptive response of the heart is diminished possibly due to a pathological activation of MMPs via nitric oxide - peroxynitrite-induced oxidative/nitrosative stress. Therefore, the aim of MMPharma consortium is to develop selective MMP inhibitors capable of reducing infarct size as a new therapeutic option against acute myocardial infarction. The consortium is based on a co-operation of 4 biotechnology companies, a technology transfer office and academic research groups using the most advanced technologies in chemoinformatics, bioinformatics, genomics, and toxicogenomics. MMPharma consortium is capable of executing a fast-reacting integrated preclinical drug-development process from target identification via generation of innovative molecular libraries and development of supporting biomedical instruments to pharmacological testing in complex animal models as well as in human clinical trials.